Juan C Fontecilla-camps

Metalloproteins: Methods and Protocols - Methods in Molecular Biology Juan C Fontecilla-camps 2014 edition

Jacket Description/Back: Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. "Metalloproteins: Methods and Protocols" addresses multiple aspects of metalloenzyme research from the production and purification of metalloproteins using both standard recombinant techniques and a cell-free system, to the electrochemical analysis of metalloproteins, as well as IR techniques, Mossbauer spectroscopy and the theoretical interpretation of metalloenzyme catalytic and redox processes, to name a few topics. Written in the successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, "Metalloproteins: Methods and Protocols" seeks to serve both professionals and novices interested in the metalloprotein field. "Table of Contents:1. Advances in Research on Metalloproteins Perry A. Frey 2. Purification of O2-Sensitive Metalloproteins Carlos Echavarri-Erasun, Simon Arragain, and Luis M. Rubio 3. Expression and Purification of NifB Protein from Aerobic and Anaerobic Sources Carlos Echavarri-Erasun, Simon Arragain, Alessandro A. Scandurra, and Luis M. Rubio 4. Techniques for the Production, Isolation and Analysis of Iron-Sulfur Proteins Jason C. Crack, Jeffrey Green, Andrew J. Thomson, and Nick E. Le Brun 5. Cell-Free Synthesis of the H-cluster: a Model for the In Vitro Assembly of Metalloprotein Metal Centers Jon M. Kuchenreuther, Stacey A. Shiigi, and James R. Swartz 6. Electrochemistry of Metalloproteins Protein Film Electrochemistry for the Study of E. coli [NiFe]-Hydrogenase-1 Rhiannon M. Evans and Fraser A. Armstrong 7. FTIR Spectroscopy of Metalloproteins Oscar Gutierrez-Sanz, Olaf Rudger, and Antonio L. De Lacey 8. Characterizing Millisecond Intermediates in Hemoproteins Using Rapid-Freeze-Quench Resonance Raman Spectroscopy Hirotoshi Matsumura and Pierre Moenne-Loccoz 9. A Practical Guide for Nuclear Resonance Vibrational Spectroscopy (NRVS) of Biochemical Samples and Model Compounds Hongxin Wang, Ercan Alp, Yoshitaka Yoda, and Stephen P. Cramer 10. Study of Metalloproteins Using Continuous Wave Electron Paramagnetic Resonance (EPR) Serge Gambarelli and Vincent Maurel 11. Mossbauer Spectroscopy Martin Clemancey, Genevieve Blondin, Jean-Marc Latour, and Ricardo Garcia-Serres 12. X-Ray Absorption Spectroscopy of Metalloproteins Jesse Ward, Emily Ollmann, Evan Maxey, and Lydia A. Finney 13. X-Ray Crystallographic Studies of Metalloproteins Anne Volbeda 14. Quantum Mechanical Methods for the Investigation of Metalloproteins and Related Bioinorganic Compounds Luca Bertini, Maurizion Bruschi, Ugo Cosentino, Claudio Greco, Giorgio Moro, Giuseppe Zampella, and Luca De Gioia 15. Density Functional Theory Broken Symmetry (DFT-BS) Methodology Applied to Electronic and Magnetic Properties of Bioinorganic Prosthetic Groups Jean-Marie Mouesca"Marc Notes: 'Metalloproteins' addresses multiple aspects of metalloenzyme research, from the production and purification of metalloproteins using both standard recombinant techniques and a cell-free system, to the electrochemical analysis of metalloproteins. The contributors also address topics such as IR techniques, Mossbauer spectroscopy and the theoretical interpretation of metalloenzyme catalytic and redox processes.