Syed Ali Raza

Advanced Laboratory Techniques in Zoology: Approaches to Generate and Analyse Biological Data Syed Ali Raza

Advance Laboratory Techniques in Zoology include Principles and applications of Centrifugation, Filtration, Microscopy (Scanning and Transmission Electron microscopes). These also include Micro-photography, Micrometry, Chromatography, Spectrophotometry, Electrophoresis (Agarose and PAGE), Southern and Northern Blotting. Advanced Techniques in Biochemistry are widely used for Extraction, Precipitation and estimation of Amino acids and Proteins, Lipids, Nucleic acids and the study of enzyme activity. Histological (sectioning and staining) and Molecular Biology techniques: Vector isolation, Gene insertion, Recombinant DNA Production, Gene amplification by PCR, DNA and vector purification, Preparation of genomic libraries and sub-cloning using plasmid vectors are widely used in Zoology. Immunological techniques like Radioimmunoassay, ELISA, Development of vaccines, Aseptic culture techniques, bacterial growth, isolation and identification of bacteria as well as Use of Statistical Methods are commonly used techniques in Zoology Research.