Faiza Saleem

Agrobacterium-mediated Transformation of Reporter Gene in Corn Faiza Saleem

Zea mays is the most economically important annual crop. A vector containing GUS reporter gene was introduce into corn via Agrobacterium mediated transformation. Transformants of different varieties of maize will be efficiently produced. The transformants will be histochemically detected for the presence of GUS reporter gene. Some conditions for best Agrobacterium mediated transformation was standardized using Agrobacterium tumefaciens strain EHA101 harboring binary vector Pzy102 containing GUS as reporter gene in kashmiri variety of corn. Numerous factors were compared and optimized for effecting the transfer of T-DNA and its incorporation to the genomic system of corn such as immature embryos of 2.5mm size were analyzed as explants of choice,100?M acetosyringone concentrations, N6 ad MS media composition, Clorox concentrations of 30%, co-cultivation durations of 3 days under dark at 22°C and bacterial concentrations was adjusted to get OD550 0.36, GUS chemical composition and incubation temperature of 37°C during GUS analysis were assessed as best. Different optimal experimental conditions were establish to enhance the ?-glucuronidase (GUS) activity after transformation.